Rambach Agar base and supplement

Productcode: RR702
Supplier: CHROMagar
Package size: 5000 ml

Product information

Despite its early discovery in the 1880’s, Salmonella remains a major worldwide pathogen and one of the most common food-borne poisons.
For instance, Salmonella has an incidence rate of 16.2 cases per 100,000 (CDC estimation, 2008).

  1. Reliable: the first commercially available (1989) chromogenic medium, is still successful today, thanks to its performance.

  2. Very high sensitivity:
    Salmonella → 93,9%*
    *Sensitivlity from scientific study: Gruenewald, R. et al. 1991. Use of Rambach Propylene Glycol Containing Agar for Identification of Salmonella spp. J.C.M. 29: 2354-2356.

  3. Intense red colouration for easy reading, compared to other chromogenic media. 

  4. Fast results: particularly useful in case of a sudden, dangerous outbreak of Salmonella food poisoning. 

  5. High specificity / Lower worlkload: The conventional media for the detection of Salmonella by H2S character has very poor specificity, creating an abundance of false positives (Citrobacter, Proteus, etc.) among the rare real positive Salmonella. The workload for unnecessary examination of suspect colonies is so high that the real positive Salmonella colonies might often be missed in routine testing. Because of their poor specificity, conventional media require tedious examination of at least 10 colonies per suspected sample. On the contrary, Rambach Agar eliminates most of those false positives and allows technicians to focus on the real contaminated samples.

Rambach Agar base and supplement

Contact our specialist

For more information from Labema’s specialist. The specialist for this product is Minni Raitio.

Send request


Comparison of Four Chromogenic Media and Hektoen Agar for Detection and Presumptive Identification of Salmonella Strains in Human Stools
We compared the performance of four commercial chromogenic media—namely, ABC medium (Lab M. Ltd., Bury, United Kingdom), COMPASS Salmonella agar (Biokar Diagnostics, Beauvais, France), CHROMagar™ Salmonella agar (CHROMagar Company, Paris, France), and SM ID agar (bioMerieux, Marcy l’Etoile, France)— with conventional Hektoen medium. Nine hundred sixteen stool samples from inpatients at three hospitals were cultured, in parallel, on the five media, both by direct inoculation and after selective enrichment in selenite broth. The specificity of the four chromogenic media was better than 91% after incubation for 24 h (77.7% with Hektoen agar) and better than 84% after incubation for 48 h (74.8% with Hektoen agar). This higher specificity reduces the need for confirmatory tests, thereby cutting technical time and reagent requirements. Both COMPASS agar and CHROMagar™ Salmonella, which after simple additional tests showed close efficiencies (96 and 97%, respectively), can be recommended as single-plate media of choice for the detection and presumptive identification of salmonellae in stools.